Real-time protein NMR spectroscopy and investigation of assisted protein folding

Kumar Amit; Balbach Jochen

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Real-time protein NMR spectroscopy and investigation of assisted protein folding

機譯：實時蛋白質NMR光譜學和輔助蛋白質折疊研究

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Background: During protein-folding reactions toward the native structure, short-lived intermediate states can be populated. Such intermediates expose hydrophobic patches and can self-associate leading to non-productive protein misfolding. A major focus of current research is the characterization of short-lived intermediates and how molecular chaperones enable productive folding. Real-time NMR spectroscopy, together with the development of advanced methods, is reviewed here and the potential these methods have to characterize intermediate states as well as interactions with molecular chaperone proteins at single-residue resolution is highlighted.

機譯：背景：在向天然結構的蛋白質折疊反應期間，可能會出現短暫的中間狀態。此類中間體會暴露疏水性斑塊，并且會自締合，導致非生產性蛋白質錯誤折疊。當前研究的主要重點是短壽命中間體的表征以及分子伴侶如何實現生產性折疊。本文回顧了實時NMR光譜技術以及先進方法的發展，并著重介紹了這些方法表征中間狀態以及與分子伴侶蛋白在單殘基分辨率下相互作用的潛力。

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《Biochimica et Biophysica Acta. General Subjects》 |2015年第10期|共8頁
作者
Kumar Amit; Balbach Jochen;
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原文格式 PDF
正文語種 eng
中圖分類 Q5;
關鍵詞
Real-time NMR; Protein folding; Kinetic intermediates; Molecular chaperones;

機譯：實時核磁共振;蛋白質折疊;動力學中間體;分子伴侶;
入庫時間 2022-08-18 09:01:40

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1. Real-time protein NMR spectroscopy and investigation of assisted protein folding [J] . Kumar Amit, Balbach Jochen Biochimica et Biophysica Acta. General Subjects . 2015,第10期

機譯：實時蛋白質NMR光譜學和輔助蛋白質折疊研究
2. Real-time monitoring of enzyme-assisted animal protein hydrolysis by NMR spectroscopy - An NMR reactomics concept [J] . LWT-Food Science & Technology . 2018,第期

機譯：NMR光譜對酶輔助動物蛋白水解的實時監測 - NMR反應組合概念
3. Very Fast Two-Dimensional NMR Spectroscopy for Real-Time Investigation of Dynamic Events in Proteins on the Time Scale of Seconds [J] . Paul Schanda, Bernhard Brutscher Journal of the American Chemical Society . 2005,第22期

機譯：實時二維核磁共振波譜，用于實時研究蛋白質的動態事件（以秒為單位）
4. Dynamics of Partially Folded and Unfolded Proteins Investigated with Quasielastic Neutron Spectroscopy [C] . Andreas M. Stadler International Conference on Quasielastic Neutron Scattering . 2018

機譯：用擬塑性中子譜研究部分折疊和展開蛋白質的動態
5. Mechanistic Investigations on Hsp70 Chaperone-Mediated Protein Folding, and, Photo-CIDNP enhancements in Heteronuclear Correlation NMR Spectroscopy. [D] . Sekhar, Ashok. 2011

機譯：Hsp70分子伴侶介導的蛋白質折疊和Photo-CIDNP增強在異核相關NMR光譜中的機理研究。
6. In-Cell NMR Spectroscopy-In vivo Monitoring of the Structure Dynamics Folding and Interactions of Proteins at Atomic Resolution [O] . Thallapuranam K Suresh Kumar, Ryan Thurman, Srinivas Jayanthi -1

機譯：內嵌NmR光譜-體內監測在原子分辨率的結構動力學折疊和蛋白質的相互作用的
7. Very Fast Two-Dimensional NMR Spectroscopy for Real-Time Investigation of Dynamic Events in Proteins on the Time Scale of Seconds [O] . -1

機譯：非常快的二維NMR光譜，用于實時調查蛋白質中動態事件的時間段落的時間段

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Real-time protein NMR spectroscopy and investigation of assisted protein folding

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