Genomic Phenotype of Pulmonary Fibroblasts in Idiopathic Pulmonary Fibrosis.

機譯：特發性肺纖維化中肺成纖維細胞的基因表型。

獲取原文

獲取原文并翻譯 | 示例

頁面導航

摘要
著錄項
相似文獻
相關主題

摘要

IPF is a fatal form of interstitial lung disease (ILD) of unknown etiology and for which there is no proven medical therapy. The median post diagnosis survival time is three years. While there are an estimated 5 million people affected by IPF world-wide, the prevalence continues to escalate. In the United States alone the incidence of IPF has increased considerably from a reported 50,000 people in 2000 to current estimates closer to 200,000. IPF is characterized by extensive parenchymal fibrosis which ultimately destroys the lungs' architecture and function. This fibrosis results from an unremitting accumulation of fibroblasts concomitant with their relentless deposition of extra cellular matrix, predominantly in the form of type 1 collagen. In light of this, the fibroblast is arguably the effector cell of the IPF phenotype. With no effective therapy available and over 40,000 individuals dying annually, as many as die from breast cancer, there is clearly an immediate need to expand our understanding of IPF, and specifically---the fibroblast. This dissertation (1) presents a novel isolation method of the pulmonary fibroblast cell population from IPF and normal lungs derived without long-term tissue culture; (2) comprehensively characterizes the genomic phenotype of these non-cultured isolated pulmonary fibroblasts in IPF in comparison to normal controls, and (3) documents the evolution and in vitro de-differentiation of this fibroblast model system. This model is then compared to the current in vitro explant-derived fibroblast model system The insights gleaned from these studies will further our understanding of the contribution of the fibroblast to the pathogenesis of IPF in addition to providing a platform for systematically investigating aberrant pathways. Moreover, the findings herein will promote and facilitate development of a more strategic approach to our investigations of this aberrant promoter of IPF progression---the fibroblast.

機譯：IPF是病因不明的致命性間質性肺病（ILD）的一種，目前尚無經過證實的醫學療法。診斷后中位生存時間為三年。盡管全世界估計有500萬人受到IPF的影響，但這種流行率仍在不斷上升。僅在美國，IPF的發病率就從2000年的50,000人增加到目前的近200,000人，大大增加了。 IPF的特征是廣泛的實質性纖維化，最終破壞了肺部的結構和功能。這種纖維化是由于成纖維細胞不斷積累，并伴之以無休止的細胞外基質（主要是1型膠原蛋白形式）沉積。因此，成纖維細胞可以說是IPF表型的效應細胞。由于沒有有效的治療方法，每年有40,000多人死于乳腺癌，與死于乳腺癌的人一樣多，顯然迫切需要擴大我們對IPF尤其是成纖維細胞的了解。本論文（1）提出了一種無需長期組織培養的從IPF和正常肺中分離肺成纖維細胞群的新方法。（2）與正常對照相比，全面表征了IPF中這些未培養的分離的肺成纖維細胞的基因組表型，并且（3）記錄了該成纖維細胞模型系統的進化和體外去分化。然后將該模型與當前體外外植體衍生的成纖維細胞模型系統進行比較。從這些研究中收集到的見解將進一步使我們進一步了解成纖維細胞對IPF發病機制的貢獻，并為系統研究異常途徑提供平臺。此外，本文的發現將促進和促進一種更具戰略意義的方法來研究這種IPF進程異常啟動子-成纖維細胞。

著錄項

作者
Emblom-Callahan, Margaret Claire.;
展開▼
作者單位

George Mason University.;

展開▼
授予單位 George Mason University.;
學科 Biology Molecular.;Biology Bioinformatics.;Biology Cell.
學位 Ph.D.
年度 2010
頁碼 318 p.
總頁數 318
原文格式 PDF
正文語種 eng
中圖分類
關鍵詞
入庫時間 2022-08-17 11:36:57

相似文獻

外文文獻
中文文獻
專利

1. Genomic phenotype of non-cultured pulmonary fibroblasts in idiopathic pulmonary fibrosis. [J] . Emblom Callahan MC, Chhina MK, Shlobin OA, Genomics . 2010,第3期

機譯：非培養性肺成纖維細胞在特發性肺纖維化中的基因表型。
2. Genomic phenotype of non-cultured pulmonary fibroblasts in idiopathic pulmonary fibrosis [J] . Margaret C.Emblom-Callahan, Mantej K.Chhina, Oksana A.Shlobin, Genomics . 2010,第3期

機譯：非培養性肺成纖維細胞在特發性肺纖維化中的基因表型
3. Tectorigenin inhibits the in vitro proliferation and enhances miR-338* expression of pulmonary fibroblasts in rats with idiopathic pulmonary fibrosis. [J] . Zhang H, Liu X, Chen S, Journal of Ethnopharmacology: An Interdisciplinary Journal Devoted to Bioscientific Research on Indigenous Drugs . 2010,第1期

機譯：Tectorigenin抑制特發性肺纖維化大鼠的體外增殖并增強肺成纖維細胞的miR-338 *表達。
4. Development and evaluation of pulmonary imaging multi-parametric response maps for deep phenotyping of chronic obstructive pulmonary disease [C] . Jonathan L. MacNeil, Dante P. I. Capaldi, Rachel L. Eddy, Biomedical Applications in Molecular, Structural, and Functional Imaging . 2019

機譯：肺成像多參數響應圖的開發與評價，用于慢性阻塞性肺疾病的深層表型
5. Correlation of Vascular Leak Measured Using Gadofosveset-Enhanced Lung Magnetic Resonance Imaging with Radiographic and Physiologic Measures of Fibrosis in Patients with Idiopathic Pulmonary Fibrosis. [D] . Liang, Lloyd. 2018

機譯：特發性肺纖維化患者使用Gadofosveset增強肺磁共振成像測得的血管滲漏與纖維化的放射學和生理學指標的相關性。
6. A monoclonal antibody to the carboxyterminal domain of procollagen type I visualizes collagen-synthesizing fibroblasts. Detection of an altered fibroblast phenotype in lungs of patients with pulmonary fibrosis. [O] . J A McDonald, T J Broekelmann, M L Matheke, 1986

機譯：I型膠原蛋白羧基末端結構域的單克隆抗體可可視化合成膠原的成纖維細胞。肺纖維化患者肺部成纖維細胞表型改變的檢測。
7. Genomic phenotype of non-cultured pulmonary fibroblasts in idiopathic pulmonary fibrosis [O] . Emblom-Callahan Margaret C., Chhina Mantej K., Shlobin Oksana A., 2010

機譯：未培養的肺成纖維細胞在特發性肺纖維化中的基因表型

站內服務

Genomic Phenotype of Pulmonary Fibroblasts in Idiopathic Pulmonary Fibrosis.

摘要

著錄項

相似文獻

相關主題